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硅酸盐通报 ›› 2018, Vol. 37 ›› Issue (5): 1509-1514.

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低温条件微生物沉积碳酸钙试验研究

童天志;缪林昌;孙潇昊;王呈呈   

  1. 东南大学交通学院岩土工程研究所,南京,210096
  • 出版日期:2018-05-15 发布日期:2021-01-18
  • 基金资助:
    国家自然科学基金(51278099,51578147)

Microbially-induced Carbonate Precipitation in Low Temperature

TONG Tian-zhi;MIAO Lin-chang;SUN Xiao-hao;WANG Cheng-cheng   

  • Online:2018-05-15 Published:2021-01-18

摘要: 研究表明微生物固化技术可用于砂土固化及混凝土材料修复等方面,但由于微生物的特殊性,其应用受到温度、营养液等环境条件的限制.选用耐低温菌种(菌种A)与巴氏芽孢杆菌,开展了微生物诱导碳酸钙沉淀(MICP)的试验研究,对比了两者在不同温度下的MICP性能.研究首先测量两个菌种在不同温度中的细菌生长繁殖特性,然后对比分析两者的脲酶活性,最后研究两者生成碳酸钙的速率和效率.研究结果表明,相比于巴氏芽孢杆菌,菌种A在低温条件下不论是生长繁殖能力还是脲酶活性都较强,同时诱导碳酸钙沉淀的速率和效率也较高.该研究成果对MICP技术在低温环境的应用研究具有指导意义.

关键词: 巴氏芽孢杆菌;低温;碳酸钙;微生物固化

Abstract: Microbially-induced Carbonate Precipitation (MICP) can be applied in sand-solidification and the repair of concrete.However, its application was fettered due to the limitation of the requirement of temperature.This study used two species of bacteria (Bacteria A and Sporosarcina pasteurii ) to conduct MICP research in several temperatures .The bacteria cultivation was conducted in various different temperatures.Precipitation of calcium carbonate research and urease activity test were also conducted with two bacteria.The results show that Bacteria A has a better reproduction in low temperature and performs better in precipitation of CaCO 3 as well as urease activity in low temperature.This research is instructive in MICP with low-temperature environment.

Key words: Sporosarcina pasteurii;low temperature;calcite precipitation;MICP

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