改性黄腐酸/凹凸棒石复合材料的制备及其抑菌性能

硅酸盐通报 ›› 2021, Vol. 40 ›› Issue (8): 2777-2783.

改性黄腐酸/凹凸棒石复合材料的制备及其抑菌性能

杨芳芳^1,2, 张倩³, 宋亚萌¹, 惠爱平^1,2, 康玉茹^1,2, 王爱勤^1,2

1.中国科学院兰州化学物理研究所,甘肃省黏土矿物应用研究重点实验室,兰州 730000;
2.中国科学院兰州化学物理研究所盱眙凹土应用技术研发中心,淮安 211700;
3.兰州大学第二医院检验医学中心,兰州 730000

收稿日期:2021-02-21 修回日期:2021-04-02 出版日期:2021-08-15 发布日期:2021-09-02
通讯作者: 王爱勤,博士,研究员。E-mail:aqwang@licp.cas.cn
作者简介:杨芳芳(1986—),女,博士,助理研究员。主要从事抗菌材料的研究。E-mail:yangff@licp.cas.cn
基金资助:
江苏省科技厅重点研发计划(BE2019071);中国科学院STS区域重点项目(KFJ-STS-QYZX-086);兰州化学物理研究所青年合作基金(552020000093)

Prepration of Modified Fulvic Acid/Attapulgite Composites and Their Antimicrobial Properties

YANG Fangfang^1,2, ZHANG Qian³, SONG Yameng¹, HUI Aiping^1,2, KANG Yuru^1,2, WANG Aiqin^1,2

1. Key Laboratory of Clay Mineral Applied Research of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
2. R&D Center of Xuyi Palygorskite Applied Technology, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Huai'an 211700, China;
3. Laboratory Medicine Center, Lanzhou University Second Hospital, Lanzhou 730000, China

Received:2021-02-21 Revised:2021-04-02 Online:2021-08-15 Published:2021-09-02

摘要/Abstract

摘要： 黄腐酸(FA)具有抑菌消炎和促进动物生长的功效。为了进一步提升FA的广谱抑菌性,以凹凸棒石(APT)为载体,通过吸附法将FA负载于APT,同时进行十六烷基三甲基溴化铵(CTAB)改性。结果表明,FA和APT主要以静电吸附形式结合,CTAB的引入实现了FA/APT复合材料的电荷调控。复合材料对大肠杆菌和金黄色葡萄球菌的最小抑菌浓度(MIC)随着CTAB添加量的增加而减小;当CTAB添加量为15%(质量分数)时,其对大肠杆菌的MIC为1 mg/mL,对金黄色葡萄球菌的MIC为0.05 mg/mL。

关键词: 黄腐酸, 凹凸棒石, 十六烷基三甲基溴化铵, 复合材料, 广谱抑菌性

Abstract: Fulvic acid (FA) has the effect of inhibiting bacteria, anti-inflammation, and promoting animal growth. In order to further improve the broad-spectrum antimicrobial properties of FA, a composite was prepared by loading FA into attapulgite (APT) through the adsorption method, meanwhile cetyltrimethylammonium bromide (CTAB) was used to modify the composite. The results show that FA and APT are mainly combined by electrostatic adsorption, and the introduction of CTAB regulates the charge of FA/APT composites. The minimum inhibitory concentration (MIC) of the composites against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) decreases with the increase of CTAB amount. When the amount of CTAB is 15% (mass fraction), the MIC is 1 mg/mL for E. coli and the MIC is 0.05 mg/mL for S. aureus.

Key words: fulvic acid, attapulgite, cetyl trimethyl ammonium bromide, composite material, broad-spectrum antimicrobial property

中图分类号:

R318.08

杨芳芳, 张倩, 宋亚萌, 惠爱平, 康玉茹, 王爱勤. 改性黄腐酸/凹凸棒石复合材料的制备及其抑菌性能[J]. 硅酸盐通报, 2021, 40(8): 2777-2783.

YANG Fangfang, ZHANG Qian, SONG Yameng, HUI Aiping, KANG Yuru, WANG Aiqin. Prepration of Modified Fulvic Acid/Attapulgite Composites and Their Antimicrobial Properties[J]. BULLETIN OF THE CHINESE CERAMIC SOCIETY, 2021, 40(8): 2777-2783.

参考文献

[1] GUSTAFSON R H, BOWEN R E. Antibiotic use in animal agriculture[J]. Journal of Applied Microbiology, 1997, 83(5): 531-541.
[2] STOKES J M, YANG K, SWANSON K, et al. A deep learning approach to antibiotic discovery[J]. Cell, 2020, 180(4): 688-702.
[3] BEN Y J, FU C X, HU M, et al. Human health risk assessment of antibiotic resistance associated with antibiotic residues in the environment: a review[J]. Environmental Research, 2019, 169: 483-493.
[4] 冯佩诗,李桥桥,江山山,等.矿源黄腐酸的生理学功能及其在畜禽生产中应用的研究进展[J].中国畜牧杂志,2020,56(10):13-17.
FENG P S, LI Q Q, JIANG S S, et al. Advances in effect of fulvic acid on livestock and poultry production[J]. Chinese Journal of Animal Science, 2020, 56(10): 13-17 (in Chinese).
[5] RODRÍGUEZ N C, URRUTIA E C, GERTRUDIS B H, et al. Antioxidant activity of fulvic acid: a living matter-derived bioactive compound[J]. Journal of Food, Agriculture and Environment, 2011, 9(3/4): 123-127.
[6] CUSACK P. Effects of a dietary complex of humic and fulvic acids (FeedMAX 15^TM) on the health and production of feedlot cattle destined for the Australian domestic market[J]. Australian Veterinary Journal, 2008, 86(1/2): 46-49.
[7] PAPPAS A C, ZOIDIS E, THEOPHILOU N, et al. Effects of palygorskite on broiler performance, feed technological characteristics and litter quality[J]. Applied Clay Science, 2010, 49(3): 276-280.
[8] CHENG Y F, XU Q, CHEN Y P, et al. Modified palygorskite improves immunity, antioxidant ability, intestinal morphology, and barrier function in broiler chickens fed naturally contaminated diet with permitted feed concentrations of fusarium mycotoxins[J]. Toxins, 2018, 10(11): 482.
[9] 陈天虎,李宏伟,汪家权,等.凹凸棒石-银纳米复合抗菌材料制备方法和表征[J].硅酸盐通报,2005,24(2):123-126.
CHEN T H, LI H W, WANG J Q, et al. Preparation and characterization of nanopalygorskite-Ag composite material[J]. Bulletin of the Chinese Ceramic Society, 2005, 24(2): 123-126 (in Chinese).
[10] 王文波,牟斌,张俊平,等.凹凸棒石:从矿物材料到功能材料[J].中国科学:化学,2018,48(12):1432-1451.
WANG W B, MU B, ZHANG J P, et al. Attapulgite: from clay minerals to functional materials[J]. Scientia Sinica (Chimica), 2018, 48(12): 1432-1451 (in Chinese).
[11] 舒展,张毅,谢虹忆,等.硅酸盐黏土矿物在抗菌方面研究进展[J].材料工程,2018,46(4):23-30.
SHU Z, ZHANG Y, XIE H Y, et al. Research progress of silicate clay minerals in antibacterial applications[J]. Journal of Materials Engineering, 2018, 46(4): 23-30 (in Chinese).
[12] HUI A P, YAN R, WANG W B, et al. Incorporation of quaternary ammonium chitooligosaccharides on ZnO/palygorskite nanocomposites for enhancing antibacterial activities[J]. Carbohydrate Polymers, 2020, 247: 116685.
[13] 阳虹,张玉贵,李永生,等.褐煤黄腐酸钾制黄腐酸的分子结构表征[J].河南理工大学学报(自然科学版),2014,33(4):539-542.
YANG H, ZHANG Y G, LI Y S, et al. Molecular structural characterization of fulvic acid made by potassium fulvic acid from lignite[J]. Journal of Henan Polytechnic University (Natural Science), 2014, 33(4): 539-542 (in Chinese).
[14] GONG G Q, YUAN X, ZHANG Y J, et al. Characterization of coal-based fulvic acid and the construction of a fulvic acid molecular model[J]. RSC Advances, 2020, 10(9): 5468-5477.
[15] FU H B, QUAN X. Complexes of fulvic acid on the surface of hematite, goethite, and akaganeite: FTIR observation[J]. Chemosphere, 2006, 63(3): 403-410.
[16] ZHANG Y, WANG W B, MU B, et al. Effect of grinding time on fabricating a stable methylene blue/palygorskite hybrid nanocomposite[J]. Powder Technology, 2015, 280: 173-179.
[17] 蔡访勤,赵曼瑞,荆宇红.黄腐酸和腐植酸的抑菌试验及其对细胞免疫应答的影响[J].河南医学院学报,1984,19(4):12-15.
CAI F Q, ZHAO M R, JING Y H. Antimicrobial test of fulvic acid and humic acid and their effects on cellular immune response[J]. Journal of Henan Medical College, 1984, 19(4): 12-15 (in Chinese).
[18] 高金岗,王锐萍,郝清玉,等.生化黄腐酸与常见抑菌药品的抑菌效果比较[J].腐植酸,2009(5):18-23.
GAO J G, WANG R P, HAO Q Y, et al. Comparison of antibacterial effect between biochemical fulvic acid and common antibacterial drugs[J]. Humic Acid, 2009(5): 18-23 (in Chinese).
[19] VAN RENSBURG C E J, VAN STRATEN A, DEKKER J. An in vitro investigation of the antimicrobial activity of oxifulvic acid[J]. Journal of Antimicrobial Chemotherapy, 2000, 46(5): 853-854.
[20] QIN Y, ZHANG M, DAI W F, et al. Antidiarrhoeal mechanism study of fulvic acids based on molecular weight fractionation[J]. Fitoterapia, 2019, 137: 104270.
[21] BAI H X, CHANG Q F, SHI B M, et al. Effects of fulvic acid on growth performance and meat quality in growing-finishing pigs[J]. Livestock Science, 2013, 158(1/2/3): 118-123.
[22] GAO Y, HE J, HE Z L, et al. Effects of fulvic acid on growth performance and intestinal health of juvenile loach Paramisgurnus dabryanus (Sauvage)[J]. Fish & Shellfish Immunology, 2017, 62: 47-56.
[23] MAO Y M. Modulation of the growth performance, meat composition, oxidative status, and immunity of broilers by dietary fulvic acids[J]. Poultry Science, 2019, 98(10): 4509-4513.
[24] WINKLER J, GHOSH S. Therapeutic potential of fulvic acid in chronic inflammatory diseases and diabetes[J]. Journal of Diabetes Research, 2018, 2018: 5391014.
[25] WANG Y Y, WANG B Z, LIU Q, et al. Palygorskite supplementation improves growth performance, oxidative status, and intestinal barrier function in cherry valley ducks[J]. The Journal of Poultry Science, 2019, 56(3): 186-194.
[26] ZHANG R Q, DU M F, WEN C, et al. Evaluation of palygorskite on pellet quality, growth, antioxidant status and mineral contents of Chinese mitten crabs (Eriocheir sinensis)[J]. Aquaculture Research, 2020, 51(4): 1446-1454.
[27] ZHANG J M, LV Y, TANG C H, et al. Effects of dietary supplementation with palygorskite on intestinal integrity in weaned piglets[J]. Applied Clay Science, 2013, 86: 185-189.
[28] 马壮,郜世喜,李智超.坡缕石性能及应用现状[J].硅酸盐通报,2011,30(3):583-587.
MA Z, GAO S X, LI Z C. Performance and application status of palygorskite[J]. Bulletin of the Chinese Ceramic Society, 2011, 30(3): 583-587 (in Chinese).
[29] CAI X, ZHANG J L, OUYANG Y, et al. Bacteria-adsorbed palygorskite stabilizes the quaternary phosphonium salt with specific-targeting capability, long-term antibacterial activity, and lower cytotoxicity[J]. Langmuir, 2013, 29(17): 5279-5285.
[30] SHA D, YANG X, WANG B L, et al. Surface grafting of a quaternary ammonium salt on macroporous polyvinyl alcohol-formaldehyde sponges and their highly efficient antibacterial performance[J]. ACS Applied Polymer Materials, 2020, 2(11): 4936-4942.
[31] TISCHER M, PRADEL G, OHLSEN K, et al. Quaternary ammonium salts and their antimicrobial potential: targets or nonspecific interactions?[J]. ChemMedChem, 2012, 7(1): 22-31.